dc.contributor.author | Budama-Kilinc, Yasemin | |
dc.contributor.author | Badur, Selim | |
dc.contributor.author | ÇAKIR KOÇ, Rabia | |
dc.contributor.author | Mes, Sevim | |
dc.contributor.author | Ozkan, Busra | |
dc.date.accessioned | 2021-03-04T11:42:00Z | |
dc.date.available | 2021-03-04T11:42:00Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Ozkan B., Budama-Kilinc Y., ÇAKIR KOÇ R., Mes S., Badur S., "Application of an immunoglobulin Y-alkaline phosphatase bioconjugate as a diagnostic tool for influenza A virus", BIOENGINEERED, cilt.10, sa.1, ss.33-42, 2019 | |
dc.identifier.issn | 2165-5979 | |
dc.identifier.other | av_73a833f2-b9e2-46b6-8385-17f84de93c97 | |
dc.identifier.other | vv_1032021 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12627/79526 | |
dc.identifier.uri | https://doi.org/10.1080/21655979.2019.1586054 | |
dc.description.abstract | The diagnosis of influenza A virus is essential since it can be confused with influenza A like illness and lead to inaccurate drug prescription. In this study, the M2e peptide, a strategic antigen that is conserved in all virus subtypes, was used as a diagnostic marker of influenza A. For the first time, M2e-specific IgY antibody was covalently conjugated to alkaline phosphatase (ALP) enzyme in the presence of glutaraldehyde. The antibody-enzyme bioconjugate was characterized by fluorescence and Fourier-transform infrared spectroscopy. Subsequently, the diagnostic value of this bioconjugate was evaluated by direct sandwich ELISA using nasopharyngeal swab samples positive/negative for H1N1 and H3N2, which were previously analyzed by rRT-PCR for influenza. In conclusion, the M2e-specific IgY-ALP bioconjugate demonstrated positive results for Influenza A in samples that were diagnosed as Influenza A via the RT-PCR method. | |
dc.language.iso | eng | |
dc.subject | Yaşam Bilimleri (LIFE) | |
dc.subject | Biyoteknoloji | |
dc.subject | BİYOTEKNOLOJİ VE UYGULAMALI MİKROBİYOLOJİ | |
dc.subject | Mikrobiyoloji | |
dc.subject | Yaşam Bilimleri | |
dc.subject | Temel Bilimler | |
dc.title | Application of an immunoglobulin Y-alkaline phosphatase bioconjugate as a diagnostic tool for influenza A virus | |
dc.type | Makale | |
dc.relation.journal | BIOENGINEERED | |
dc.contributor.department | Yıldız Teknik Üniversitesi , , | |
dc.identifier.volume | 10 | |
dc.identifier.issue | 1 | |
dc.identifier.startpage | 33 | |
dc.identifier.endpage | 42 | |
dc.contributor.firstauthorID | 260448 | |