dc.contributor.author | Cavusoglu, Kader | |
dc.contributor.author | Unal, Duri Şehvar | |
dc.contributor.author | Ozturk, Nuri | |
dc.contributor.author | Seker, Tuncay | |
dc.contributor.author | Aslan, Tolga | |
dc.contributor.author | Duru, Adil Doganay | |
dc.contributor.author | Saygili, Tahsin | |
dc.contributor.author | Hoxhaj, Gerta | |
dc.contributor.author | Hiz, Mahmut Can | |
dc.contributor.author | Birgul-Iyison, Necla | |
dc.contributor.author | Ozturk, Mehmet | |
dc.contributor.author | Koman, Ahmet | |
dc.contributor.author | Kavak, Ersen | |
dc.contributor.author | Najafov, Ayaz | |
dc.date.accessioned | 2021-03-04T11:52:49Z | |
dc.date.available | 2021-03-04T11:52:49Z | |
dc.date.issued | 2010 | |
dc.identifier.citation | Kavak E., Najafov A., Ozturk N., Seker T., Cavusoglu K., Aslan T., Duru A. D. , Saygili T., Hoxhaj G., Hiz M. C. , et al., "Analysis of the Wnt/B-catenin/TCF4 pathway using SAGE, genome-wide microarray and promoter analysis: Identification of BRI3 and HSF2 as novel targets", CELLULAR SIGNALLING, cilt.22, sa.10, ss.1523-1535, 2010 | |
dc.identifier.issn | 0898-6568 | |
dc.identifier.other | vv_1032021 | |
dc.identifier.other | av_7485465a-8af2-4537-8bf2-8635d254cc30 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12627/80088 | |
dc.identifier.uri | https://doi.org/10.1016/j.cellsig.2010.05.021 | |
dc.description.abstract | The Wnt signaling pathway is involved in many differentiation events during embryonic development and can lead to tumor formation after aberrant activation of its components. beta-catenin, a cytoplasmic component, plays a major role in the transduction of canonical Wnt signaling. The aim of this study was to identify novel genes that are regulated by active beta-catenin/TCF signaling in hepatocellular carcinoma-derived Huh7 cells with high (transfected) and low beta-catenin/TCF activities. High TCF activity Huh7 cells led to earlier and larger tumor formation when xenografted into nude mice. SAGE (Serial Analysis of Gene Expression), genome-wide microarray and in silico promoter analysis were performed in parallel, to compare gene expression between low and high beta-catenin/TCF activity clones, and also those that had been rescued from the xenograft tumors. SAGE and genome-wide microarray data were compared and contrasted. BRI3 and HSF2 were identified as novel targets of Wnt/beta-catenin signaling after combined analysis and confirming experiments including qRT-PCR, ChIP, luciferase assay and lithium treatment. (C) 2010 Elsevier Inc. All rights reserved. | |
dc.language.iso | eng | |
dc.subject | Yaşam Bilimleri | |
dc.subject | Moleküler Biyoloji ve Genetik | |
dc.subject | Temel Bilimler | |
dc.subject | Sağlık Bilimleri | |
dc.subject | Temel Tıp Bilimleri | |
dc.subject | Histoloji-Embriyoloji | |
dc.subject | Tıp | |
dc.subject | Yaşam Bilimleri (LIFE) | |
dc.subject | Moleküler Biyoloji ve Genetik | |
dc.subject | HÜCRE BİYOLOJİSİ | |
dc.title | Analysis of the Wnt/B-catenin/TCF4 pathway using SAGE, genome-wide microarray and promoter analysis: Identification of BRI3 and HSF2 as novel targets | |
dc.type | Makale | |
dc.relation.journal | CELLULAR SIGNALLING | |
dc.contributor.department | Boğaziçi Üniversitesi , , | |
dc.identifier.volume | 22 | |
dc.identifier.issue | 10 | |
dc.identifier.startpage | 1523 | |
dc.identifier.endpage | 1535 | |
dc.contributor.firstauthorID | 2207849 | |