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dc.contributor.authorSarikaya, Aysegul Topal
dc.contributor.authorArda, Nazli
dc.contributor.authorGhods, Farinaz Jafari
dc.contributor.authorHamuryudan, Vedat
dc.date.accessioned2021-03-04T14:57:12Z
dc.date.available2021-03-04T14:57:12Z
dc.date.issued2017
dc.identifier.citationGhods F. J. , Sarikaya A. T. , Arda N., Hamuryudan V., "MiRNA and mRNA Profiling in Systemic Lupus Reveals a Novel Set of Cytokine - Related miRNAs and their Target Genes in Cases With and Without Renal Involvement", KIDNEY & BLOOD PRESSURE RESEARCH, cilt.42, sa.6, ss.1322-1337, 2017
dc.identifier.issn1420-4096
dc.identifier.othervv_1032021
dc.identifier.otherav_843b8bfb-4f3b-44f4-8e5d-d5118812cefe
dc.identifier.urihttp://hdl.handle.net/20.500.12627/89970
dc.identifier.urihttps://doi.org/10.1159/000485987
dc.description.abstractBackground/Aims: MiRNAs transpire as promising elements in molecular medicine for the identification of new diagnostic, prognostic and targeting therapeutic biomarkers. This study consisted of four steps: First, to investigate one or a group of specific diagnostic miRNAs for Systemic Lupus Erythematosus (SLE) disease in patients with and without renal involvement, second, to identify cytokines genes' expression profiling, third, comparing the profiles with related amounts in the serum and finally, to study target-gene-mediated functional roles of miRNAs, which have been correlated to disease development and progression. Methods: In order to use in microarray assays total RNA and miRNAs were isolated from blood and serum samples that were obtained from 16 SLE patients (9 with renal involvement and 7 without renal involvement). Taking coexistence of factors such as hypocomplementemia, positive ANA and anti-DNA into account, obtained data were processed. For each differentially expressed miRNA, potential target genes were predicted by microRNAorg, TargetScan and PITA prediction tools. Obtained mRNA profiling data were interrogated for the target genes. MiRNA and mRNA microarray results were confirmed by QRT-PCR. Finally, the amounts of cytokines were measured by multiplex ELISA method. Results: The results of study showed that among differentially expressed miRNAs in SLE patients with renal involvement compared to those without renal involvement, hsa-miR-766-3p, may play pivotal roles in PI3K-AKT-mTOR pathway. In addition according to the obtained data it is suggested that blood-borne proinflammatory cytokines such as IL-4, IL-6 and TNF-alpha alongside with disease stage and severity may contribute to this differential expression of these miRNA which may be leading to insulin resistance. Finally, hsa-miR-621, which was differentially expressed in hypertensive SLE patients without renal involvement and a positive ANA test with its predicted target gene Kallikrein-related peptidase 9 '' may play a role in the pathophysiology of hypertension in SLE. Conclusions: We reported some human miRNAs which were differentially expressed in SLE patients according to disease activity and renal involvement. Larger studies are necessary to confirm our findings and detect further biomarkers. (c) 2017 The Author(s) Published by S. Karger AG, Basel
dc.language.isoeng
dc.subjectTıp
dc.subjectSağlık Bilimleri
dc.subjectTemel Tıp Bilimleri
dc.subjectBiyokimya
dc.subjectFizyoloji
dc.subjectDahili Tıp Bilimleri
dc.subjectİç Hastalıkları
dc.subjectNefroloji
dc.subjectYaşam Bilimleri
dc.subjectTemel Bilimler
dc.subjectKlinik Tıp (MED)
dc.subjectPERİFERAL VASKÜLER HASTALIĞI
dc.subjectKlinik Tıp
dc.subjectÜROLOJİ VE NEFROLOJİ
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectBiyoloji ve Biyokimya
dc.subjectFİZYOLOJİ
dc.titleMiRNA and mRNA Profiling in Systemic Lupus Reveals a Novel Set of Cytokine - Related miRNAs and their Target Genes in Cases With and Without Renal Involvement
dc.typeMakale
dc.relation.journalKIDNEY & BLOOD PRESSURE RESEARCH
dc.contributor.departmentİstanbul Yeni Yüzyıl Üniversitesi , ,
dc.identifier.volume42
dc.identifier.issue6
dc.identifier.startpage1322
dc.identifier.endpage1337
dc.contributor.firstauthorID39686


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