The Effect of Dietary Zinc (II) Chelate and Zinc (II) Enriched Soybean Meal on Selected Parameters of In vitro Caecal Fermentation of Laying Hens

Abstract

Soybean meal was enriched with Zn (II) ions via biosorption using inorganic salt (ZnSO4 center dot 7H(2)O). Biosorption was conducted using a column reactor with a bed of a volume of 0.1 dm(3). The biosorption process was conducted at a temperature of 20 degrees C to bed saturation, controlling the concentration of solution flowing out of the column The enriched biomass was air dried for 48 h. The control group (C) and two experimental groups (I and II) were distinguished. The availability of zinc from the preparation obtained via biosorption method was examined in the first group (I) while the availability of zinc in a from organic chelate was assessed in the second group (II). The caecum was collected during dissection and the ingesta was obtained from it After incubation, the gas contained in the serum bottles was subjected to analysis in order to examine bacteria activity in the ingesta. The analysis of the produced hydrogen and methane were conducted using gas chromatography method on a gas chromatograph. The samples of liquid ingesta were subjected to analysis using a gas chromatograph (Agilent Technologies 7890A GC System) with an FID detector in order to determine total SCFA concentration and the percentage contribution of particular acids: acetic, propionic, isobutyric, butyric, isovaleric and valeric. The results analysis proved no negative influence of soybean meal enriched with zinc using biosorption method on in vitro fermentation process in caecum.