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dc.contributor.authorAGUS, N.
dc.contributor.authorBozcal, Elif
dc.contributor.authorOner, O.
dc.contributor.authorUZEL, ATAÇ
dc.contributor.authorYILMAZ, N. O.
dc.date.accessioned2021-03-05T07:14:11Z
dc.date.available2021-03-05T07:14:11Z
dc.date.issued2013
dc.identifier.citationYILMAZ N. O. , AGUS N., Bozcal E., Oner O., UZEL A., "Detection of plasmid-mediated AmpC beta-lactamase in Escherichia coli and Klebsiella pneumoniae", INDIAN JOURNAL OF MEDICAL MICROBIOLOGY, cilt.31, ss.53-59, 2013
dc.identifier.issn0255-0857
dc.identifier.otherav_92b830eb-5f73-4a25-8192-70b0aa868843
dc.identifier.othervv_1032021
dc.identifier.urihttp://hdl.handle.net/20.500.12627/98938
dc.identifier.urihttps://doi.org/10.4103/0255-0857.108723
dc.description.abstractBackground: Detecting plasmid-mediated AmpC (pAmpC). beta-lactamase-producing organism is important for optimal infection control and providing accurate and effective treatment option for physicians. Objectives: The aim of this study was to investigate the prevalence of pAmpC. beta-lactamase and compare the results of boronic acid (BA) disk test with other phenotypic tests detecting AmpC positive isolates. Materials and Methods: A total of 273 clinical isolates of Klebsiella pneumoniae (n: 82) and Escherichia coli (n: 191) were analysed. The presence of pAmpC. beta-lactamase was determined by BA disk test, cefoxitin (FOX) screening test, modified three dimensional test (M3DT), and multiplex polymerase chain reaction (PCR). Pulsed-field gel electrophoresis was performed to evaluate the genetic similarities between isolates. To detect extended spectrum. beta-lactamases (ESBL) in the presence of AmpC. beta-lactamase, ESBL confirmation test was carried out with and without BA solution. Results: Of the 273 strains tested, 127 strains were found FOX resistant, 114 were positive by M3DT, 108 were positive in BA disk test, and the multiplex PCR detected 24 pAmpC. beta-lactamase-positive isolate. The prevalence of AmpC-producing strains was 10.9% in E. coli and 3.6% in K. pneumoniae in the tested population by PCR. CIT and MOX group genes were predominant type in these strains. Conclusion: These results emphasize that clinical laboratories should consider testing the presence of pAmpC enzymes particularly in FOX-resistant isolates, and BA disk test will improve detection of this emerging resistance phenotype.
dc.language.isoeng
dc.subjectYaşam Bilimleri
dc.subjectİmmünoloji
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectTemel Bilimler
dc.titleDetection of plasmid-mediated AmpC beta-lactamase in Escherichia coli and Klebsiella pneumoniae
dc.typeMakale
dc.relation.journalINDIAN JOURNAL OF MEDICAL MICROBIOLOGY
dc.contributor.departmentIzmir Tepecik Training & Research Hospital , ,
dc.identifier.volume31
dc.identifier.issue1
dc.identifier.startpage53
dc.identifier.endpage59
dc.contributor.firstauthorID93764


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