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dc.contributor.authorSarikaya, AT
dc.contributor.authorTemizkan, G
dc.contributor.authorAkman, G
dc.date.accessioned2021-03-05T07:20:03Z
dc.date.available2021-03-05T07:20:03Z
dc.date.issued2006
dc.identifier.citationSarikaya A., Akman G., Temizkan G., "Nickel resistance in fission yeast associated with the magnesium transport system", MOLECULAR BIOTECHNOLOGY, cilt.32, ss.139-145, 2006
dc.identifier.issn1073-6085
dc.identifier.otherav_93435587-d4c3-47fa-9c77-31e78fb52148
dc.identifier.othervv_1032021
dc.identifier.urihttp://hdl.handle.net/20.500.12627/99266
dc.identifier.urihttps://doi.org/10.1385/mb:32:2:139
dc.description.abstractWe isolated and characterized a nickel (Ni2+)-resistant mutant (GAI) of Schizosaccharomyces pombe. This mutant strain displayed resistance to both Ni2+ and Zn2+, but not to Cd2+, Co2+, and CU2+. The growth rate of GAI increased proportionally with increasing Mg2+ concentrations until 50 nM Mg2+. The GAI mutation phenotype Suggests a defect in Mg2+ uptake. Sequence analysis of the GAI open reading frame (ORF) 013779, which is homologous to the prokaryotic and eukaryotic CorA Mg2+ transport systems, revealed a point mutation at codon 153 (ccc to ace) resulting in a Pro 153Thr substitution in the N-terminus of the CorA domain. Our results provide novel genetic information about Ni2+ resistance in fission yeast. Specifically, that reducing Mg2+ influx through the CorA Mg2+ transport membrane protein confers Ni2+ resistance in S. pombe. We also report that Ni2+ ion detoxification of the fission yeast is related to histidine metabolism and pH.
dc.language.isoeng
dc.subjectSitogenetik
dc.subjectTemel Bilimler
dc.subjectYaşam Bilimleri
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectBiyoteknoloji
dc.subjectMikrobiyoloji
dc.subjectBİYOTEKNOLOJİ VE UYGULAMALI MİKROBİYOLOJİ
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectBİYOKİMYA VE MOLEKÜLER BİYOLOJİ
dc.titleNickel resistance in fission yeast associated with the magnesium transport system
dc.typeMakale
dc.relation.journalMOLECULAR BIOTECHNOLOGY
dc.contributor.department, ,
dc.identifier.volume32
dc.identifier.issue2
dc.identifier.startpage139
dc.identifier.endpage145
dc.contributor.firstauthorID177765


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